Abstract : SARS-CoV-2, or severe acute respiratory syndrome has spread to every nation in the world. Global interest has been focused on finding a cure and creating an effective coronavirus vaccine as a result of the development of this new coronavirus. To analyse the genetics of the SARS-CoV2 Omicron variant in covid-19 patients and determine the primary clade that predominated in Iraq. Two patients of covid-19 were chosen to recognize Omicron strain and differentiate from Wuhan reference strain by using primer target Spike gene for study of main clade that predominant in Iraq. A total of 100 nasopharyngeal samples collected between January 2021 and January 2022. Two specific primers were used to amplify of the spike's positive area for two patients. Sequencing of positive 4 amplicon from 2 patient of sever acute respiratory syndrome isolated from male and female in the city of Al- Diwaniyah, Iraq. A unique mutations were discovered in Iraqi isolate when compared to the complete genome sequence of the SARS-CoV-2, recorded and taken as their accession number for first patient MZ411577.1, OL454752.1, MZ411580.1, and MZ477003.1. Phylogenetic analysis of each patient showed that the first patient was alignment with NC_045512.2 Wuhan reference strain with identity 100% without any mutation during period of January of 2021 to the march of the same year patient that isolated in January 2022 showed that aligned to the OL672836.1 Omicron BA.2 according to Sars-CoV-2 circulating variants viral Zone with identity 100% .with huge mutation " H69del V70del A67V (69) N211del L212I ins214EPE, G339D, N679K(674) P681H(674), N764K D796Y, T95I G142D V143del, Y144del (143)and Y145del (143). Multiple variants have been introduced as a result of the SARS-ongoing CoV-2's evolution. The VOC is causing waves of severe infection, and the new Omicron strain might make them worse.