Enterococcus faecalis bacteria are often found in cases of root canal treatment failure. Information on the antibacterial potential of Stevia rebaudiana Bertoni herbs against E.faecalis, is still very limited. To determine the antibacterial potential of Stevia rebaudiana Bertoni leaf extract against Enterococcus faecalis. In vitro laboratory experimental research with post test only design with control group. Stevia leaf extraction using maceration method. The antibacterial activity of Stevia leaf extract against Enterococcus faecalis was tested by agar well diffusion method. A total of 50 µl of Stevia leaf extracts in different concentration with positive control NaOCL 2.5% and negative control distilled water, were put into the wells on Nutrient agar plates that have been inoculated with E.faecalis. Incubate at 37℃ for 24 hours. Zone of inhibition (ZOI) was measured using a caliper. The ZOI of all test concentrations of Stevia leaf extract were varied, with a maximum diameter of 23.07 mm and a minimum of 10.34 mm, significantly different (P<0.05) from the negative control. The growth inhibition of E. faecalis bacteria by 100% and 50% Stevia leaf extracts was not significantly different (P>0.05) compared to the positive control while 25%, 12.5%, 6.25%, 3.125% and 1,56% Stevia leaf extracts were significantly different (P<0.05) compared to the positive control. All test concentrations of Stevia leaf extract have the potential to inhibit the growth of Enterococcus faecalis bacteria. Stevia leaf extracts of 100% and 50% have the ability to inhibit E.faecalis bacteria which is equivalent to NaOCl 2.5%.