Hyperglycemia in type 2 diabetic triggers the accumulation of reactive oxygen species (ROS) and there’s a decrease of antioxidant level in the blood that triggers complications. Malondialdehyde (MDA) test is the most simple and reliable test for detecting oxidation. The aim of this study is to analyze urinary MDA level in subjects with diabetes mellitus type 2. This study was conducted using cross sectional analyzis with 103 subjects in which 72 subjects has diabetes mellitus type 2 and 31 subjects were healthy. The data was observed by measuring body weight, height, ad random glucose test and MDA testing in urine. The subjects with DM type 2 has urinary MDA level higher than those healthy individuals (14,87 vs 2,92 μM). There were no significant differences of urinary MDA levels in type 2 diabetes patients with the onset of diabetes, nutritional status and the antidiabetic drugs used. There is a strong correlation between urinary MDA levels and ad random blood sugar levels in subjects with diabetes and healthy subjects (r = .690, p <0,001). This study showed that individuals with type 2 diabetes mellitus has oxidative stress and it can be determined by high MDA levels in urine compares to healthy individuals. Urinary MDA levels can be detected using spectrophotometer. It is a simple, fast, non-invasive and easy method to determine MDA levels in urine so it can be used for further research with large sample sizes.