Abstract : Primary open angle glaucoma (POAG) is the most common form of glaucoma. The risk of developing glaucoma in women is influenced by several factors, including sex hormones. Estrogen is proven to protect against the damage from glaucoma. Estrogen monotherapy is suggested to associate with a small but significant reduction in intraocular pressure (IOP) in postmenopausal women. To determine the relationship between estradiol levels and central cornea thickness (CCT) in POAG. A case control study conducted at Ophthalmic Clinic in Universitas Sumatera Utara Hospital and Network Hospital, from February 2022 until fulfilment of sample number. The samples were women with POAG, who met criteria inclusion and exclusion. Data were collected and analyzed. From 30 subjects, the mean age of POAG grup was 55.87 years, with the youngest and the oldest being 40- and 85-year-old respectively. There was no significant relationship among age category (p=0.573), as well as IOP, visual field defects, cup disc ratio and CCT, with estradiol levels in both POAG and control groups (p>0.05). Central thickness was not related to estradiol levels in POAG patients.