This study aims to investigate the effect of cholecystectomy on some biochemical parameters of women in Mosul city using serum samples. The women were divided into four groups: the first group, women who had their Cholecystectomy in premenopausal stage while the second group, women who had their Cholecystectomy in the postmenopausal stage. Third and fourth groups were healthy premenopausal and postmenopausal women respectively. Each group included 30 women. By using different techniques, lipid profile levels, alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT) activities, total bilirubin and C-reactive protein were analyzed. The results showed a significant increase (P<0.05) in lipid levels of patients groups compared to control groups. Also, the activities of gamma-glutamyl transferase and alkaline phosphatase increased significantly(P<0.05) after cholecystectomy compared to control groups. As for the levels of total bilirubin, it was very similar (P>0.05) between patients and control groups. After cholecystectomy, the results showed a significant increase (P<0.05) in C-reactive protein level compared to control groups. It was concluded from this study, that food and life styles as well as that overweighr may consider as risk factors lead to increment of liver enzymes activities and C-reactive protein level after cholecystectomy in patients women.