Dengue Hemorrhagic Fever is still a health problem in Indonesia, Palembang City, South Sumatra Province, Indonesia is one of the DHF endemic cities in Indonesia is a densely populated city and environmental conditions support vector development. Mosquito Aedes aegypti is the primary vector in the transmission of DHF. This study aims to analyze the relationship between population, population density, occupancy density and free larvae index on cases of fever and incidence of bleeding in Palembang City and can be used as a basis for DHF control in Palembang City. This study is an analytic epidemiological study with a cross-sectional design. The independent factors are population, population density, occupancy density and larvae free rate, while the dependent variable is dengue fever cases. The results of the study population, population density significantly and positively correlated with the incidence of dengue hemorrhagic fever. Meanwhile, the larvae free number has a significant effect and has a negative correlation. Population, population density and mosquito-free rate significantly affect the incidence of dengue fever in Palembang City. Population density, urban and settlement problems can be attributed to the high incidence of dengue fever indicating that population problems should be considered in every plan and assessment of urban and settlement development by the government and the private sector. DHF prevention and control programs will probably work efficiently if demographic issues, environmental conditions, and parameters free larvae index integrated into one system that analyzes and maps the distribution of DHF cases.