The aim of the present interventional study was to determines the effects of diethyleneglycol (DEG) with or without vitamin C on liver and kidney in rabbits and to study the defending properties of vitamin C against DEG induced changes. Liver and kidney function tests were carried out for 18 rabbits who had divided into 3 groups as follow: T-Group: rabbits were given (DEG) (40 mg/kg body weight/day) for 14 days and were sacrificed on the 14th day. TC-Group: rabbits were given DEG (40 mg/kg body weight/day) with vitamin C (10mg/kg/day) for 14 days and sacrificed on the 14th day. C-Group: rabbits were maintained on standard diet and water only. Venous blood samples (5ml) were withdrawn from experimental rabbits via jugular vein during animal sacrificing and left until set to clot, centrifuged, and serum collected and kept at -20 ˚C until analysis of the Liver and kidney functions including Alkaline Phosphatase, GOT, GPT, Creatinine, Urea, using special kits. Significant increase in serum levels of alkaline phosphatase (ALP), aminotransferase GOT, GPT, and urea and creatinine in the treatment groups compared to the other groups. DEG (40 mg/kg/day) for 14 days can result marked changes in serum biochemical markers of liver and kidney in rabbits.