Hypercholesterolemia is a condition with increased total and LDL cholesterol concentrations, and pumpkin seed extract can be used as a functional food to reduce this complication. The flavonoids, phytosterols, fiber, and antioxidants in pumpkin seed decrease the total cholesterol and LDL levels while increasing HDL. This study aimed to analyze the effect of pumpkin (Cucurbita moschata) seed capsule compared to simvastatin on total cholesterol, LDL, and HDL levels in hypercholesterolemic patients. A quasi-experimental method was adopted with a non-randomized control group pretest-posttest design. A total of 61 respondents were divided into the intervention and control groups. The collected data were analyzed by univariate and bivariate analysis. The results showed a decrease in total cholesterol and LDL levels and a significant increase in HDL in the control group, which was given simvastatin with a p-value of 0.000 each. This was also reported in the intervention group, which was given pumpkin seed capsules with p-values of 0.000, 0.002, and 0.000. However, the decrease in total cholesterol and LDL and the increase in HDL were more common in the control group. Pumpkin seed capsule can significantly reduce total and LDL cholesterol levels and increase HDL, but the reduction is lower than simvastatin.