This experimental work was focused to study the anti-oxidant and anti-microbial potential of Vaishvanara Churna– a polyherbal traditional ayurvedic formulation generally recommended for rheumatism (amavata), flatulence with gurgling sound (adhmana), abdominal lumps (gulma), duodenal ulcer (parinamasula), heart disease (hardya roga), etc. In order to establish the scientific pieces of evidence, the formulation was evaluated for anti-microbial potential using some gram-positive (S. aureus, Clostridium sporogenes, Bacillus subtilus.), gram-negative (Escherichia coli, Klebsiella pneumoniae, Shigella boydii, Pseudomonas aeruginosa) bacterial and fungal strains such as Candida albicans, Aspergillus brasiliensis. Additionally, the lyophilized hydroalcoholic Extract (LHE) was evaluated for antioxidant potential using 2, 7-dichlorofluorescein diacetate (DCF-DA) by employing macrophage J774A.1 cell lines and 2, 2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) method. Several fractions of the polyherbal formulation Vaishvanara Churna (5-100 mg/ml) was found to be significant antimicrobial potential against selected bacterial and fungal strains except Aspergillus brasiliensis. The antioxidant efficacy of the LHE was also found to be significant in both of the selected methods. Conclusively, this research study established the scientific evidence for the antibacterial, antifungal and antioxidant potential of Vaishvanara Churna formulation supporting its traditional therapeutic use.