Superficial mycoses are widespread skin and integument infections in sub-Saharan Africa, including Gabon. The purpose of this prospective study was to determine the prevalence of fungal agents diagnosed in superficial skin infections among patients attending the Department of Parasitology-Mycology and Tropical Medicine at the University of Health Sciences in Owendo (Gabon). The search for fungal agents was performed on all parts of the body, from the scalp to the sole of the foot. Samples were taken by squama scratching and nail cutting. Fungal agents were diagnosed by direct examination or by culture in appropriate media. Of a total of 141 patients received in consultation, most were female (52%). The mean age of patients was 36.7 years, and the most represented age group was 20-30 years with 28%. Fungal agents were found on the scalp (19.1%), inguinal fold (15.6%) and in the plantar interdigital space (14.9%). The most common clinical signs in patients were pruritus (61.0%) and desquamation (29.8%). Yeasts (59.6%), Malassezia sp (22.7%) and Candida albicans (14.2%) were the most common fungal group and species identified in patients. Onychomycosis and intertrigo of large folds were the most frequent skin disorders found in patients (21.3% each) and were mainly due to yeasts (Onychomycosis 50.0%, intertrigo 66.7%). Measures to prevent fungal contamination should be implemented to control onychomycosis (aeration of the feet), intertrigo of small folds (reduce the wearing of tight clothing) and yeasts (moderate use of facial oils).