Two kinds of antibacterial and antifungal commercial wall paints were purchased from Baghdad markets. Six isolates of microorganisms (bacteria and fungi) were isolated from two locations of ancient house walls. The viable count of microorganisms was conducted before and after using the paints. Five kinds of vegetables (cabbage, cucumber, beet, turnip and cauliflower) were selected randomly and collected from Baghdad markets for isolation of lactic acid bacteria (LAB). The antimicrobial activity of crude bacteriocin (CB) against test pathogens was performed by well diffusion assay. Partial purified bacteriocin (PPB) of Leuconostoc mesenteroides isolated from cabbage (CA) was tested against the microorganisms isolated from walls. Antibacterial and antifungal paints inhibited the growth of Escherichia coli and fungi on both wall locations. PPB of L. mesenteroides showed a significant inhibitory activity against E. coli, Aspergillus spp. and Penicillium spp. in vitro overcoming the antibacterial and antifungal commercial paints. So, it could be used as a safe alternative to chemical antibacterial and antifungal agents existed in paints.