Escherichia coli is the main cause of urinary tract infection (UTI), with the potential of its multidrug-resistant (MDR) capability. To isolate and identify E. coli implicated in UTIs and to evaluate the prevalence of MDR E. coli with its drug resistance patterns among patients. Four hundred thirty urine samples were collected from patients with UTIs between December 2021 to February 2022. A general urine examination (GUE) was performed to evaluate physical, chemical, and biological parameters. E. coli was identified according to standard microbiological methods. The total samples of E. coli (100 isolates; 50 from males and 50 from females) were evaluated by antibiotic sensitivity test using Kirby-Bauer’s disk diffusion technique. A total of 9 resistance patterns among 100 E. coli isolates were determined in patients. According to the resistance level, 46% of male and 30% of female isolates were resistant to 8≥ antibiotics. E. coli isolates were susceptible to ETP (94%), followed by AK (93%), IPM (88%), MEM (83%), NIT (81%), and GM (70%). High resistance to AMP (96%), followed by CZ (81%), CTX (79%), NA (78%), and CEF (74%), were seen for isolates. Of 100 E. coli isolates, the prevalence of MDR was 92%, while non-MDR was 8%. The prevalence of MDR in males was 98%, whereas non-MDR was 2%. The prevalence of MDR in females was 86%, whereas non-MDR was 14%. The prevalence of MDR between male and female patients was non-significant (P>0.05). E. coli isolates presented a dangerous level of MDR to recommended clinical antibiotics with high prevalence among patients.