Testicular torsion is a condition that manifests with acute pain in childhood and adolescence which can lead to infertility despite urgent surgical intervention. The aim of this study is to investigate and compare the protective and preventive effects of sufentanil, remifentanil and fentanyl, a potent analgesic agent on testicular ischemia-reperfusion injury (tIR). 28 adult male Wistar rat were divided into four groups (n=7). Torsion was performed on all left testicles rat 720' clockwise. 1 hour ischemia. 30 minutes before detortion, control group (Nacl 0.9%), fentanyl group (2 mcg/kg), Remifentanil group (1 mcg/kg), Sufentanil group (0.6 mcg/kg) were injected intraperitoneally. During 1 hour of ischemia, the left testes was detorted to restore physiology. 24 hours after reperfusion, rats in euthanasia were taken blood to see Malondialdehyde levels and ipsilateral testes to find germ cell damage. Leydig cells count and percentage tubular necrosis. Administration of Sufentanil before detortion significantly reduced malondialdehyde levels (6.01+0.24SD,P<0.05), damage to germ cells (5.93,P<0.05), Leydig cells count (20.2+4.5SD,P<0.05) and tubular necrosis (3.95+0.62SD,P<0.05) which were subjected to torsion compared to the fentanyl and remifentanil groups. However, lipid peroxidation content of malondialdehyde was significantly decline in remifentanil group (3.58+0.23SD,P<0.05). Sufentanil in ischemia preconditioning has the most protective effect compare to remifentanil by preventing testicular ischemia reperfusion injury leading to infertility in the future.