Resveratrol has antioxidant, anti-inflamation, and neuroprotective effects in oxidative stress induced by ischemic reperfusion injury in eyes with IOP elevation. This study aimed to investigate the protection effect of resveratrol against oxidative stress in retinal ganglion cells. Retinal ischemia was induced by increasing intraocular pressure to 110 mmHg for 60 minutes in 20 adult male Rattus Norvegicus rats. Resveratrol (RSV) suspension of 100M in 2L PBS was injected intravitreally 3 hours after reperfusion. After 7 days, rat’s eye was enucleated. Primary SOD-1 antibody (Santa Cruz Biotechnology, Inc.) and MDA antibody (Biorbyt Ltd.) was used to determine the SOD-1 and MDA expression in retinal ganglion cells. SOD1 expression was significantly decreased between control group and both of ischemic group (p value <0.001) and MDA level was increased after ischemic-reperfusion (IR) injury (p value <0.01). Comparison between IR+RSV group and both of IR groups was increase significantly: IR0 group (p value <0.05); IR7 group (p value <0.01), respectively. There was also significant decreased of MDA expression between resveratrol group (IR+RSV) and IR0 (p=0.030), also IR7 group (p=0.006) respectively. Resveratrol protects retinal ganglion cells from oxidative stress after IR injury by increasing SOD1 expression as antioxidant enzyme and then reducing malondialdehyde as oxidative stress marker.