About 4-8% of women of reproductive age suffer from polycystic ovary syndrome (PCOS), also known as Stein-Leventhal syndrome, a diverse endocrine condition. Research the impact of Plantago major leaves extract on the induced polycystic ovarian syndrome in rats, assess the impact of Plantago major leaves extract on biochemical markers (Adiponectin and serum blood glucose), and examine the impact of Plantago major leaves extract on hormones (FSH, LH, testosterone, and estrogen), and contrast the impact of Plantago major leaves extract with that of metformin. Female rats were treated for 21 days with 0.4 mL of oral letrozole (5.3 mg/kg) before receiving a dose of Metformin (500 mg/kg) for the first group, a high dose of Plantagomajor (1500 mg/kg) for the second group, a low dose of Plantagomajor (1000 mg/kg), and a combination of Plantagomajor (500 mg/kg) and metformin (250 mg/kg) for the third group. Compared to the induction group, the plantagomajor treated group had reduced levels of glucose, testosterone, and LH, but higher levels of adiponectin, estradiol, and FSH. In comparison to metformin, plantago major treatment groups saw greater effects in terms of easing PCOS symptoms in female rats.