Abstract : The emergence of new coronavirus has attracted global attention to work towards finding a treatment and developing an effective vaccine against the virus. Spike protein of SARSCoV-2 mediates viral entry into host cells by binding ACE2 through the receptor binding domain (RBD). Was to highlight the sequencing of gene variants of spike for SARS-CoV2 virus in Iraqi isolates. A total 100 nasopharyngeal samples of COVID-19 were collected from period of July of 2021 to January 2022 in Al-diwanyah city, Iraq. To determine the main clade predominant in Iraq, set of five primer, target sequence parts of spike gene of SARS-CoV2 were used. The results of amplification of positive region of spike for nine patients revealed sequencing of eight positive of SARS-COV-2 and distinct mutations were found in local isolates compared to the full genome sequence of the SARS-COV-2 Wuhan strain. Accession number was recorded in NCBI locus for first patient ON908701.1 ON908700.1 ON908699.1 OL477012.1, ON908698.1. phylogenetic analysis of each patient showed alignment with MW966601.1 which had reference for Kappa cladeB.1.617.1, and identical 100% to the ON668147.1. with mutation in amino acid E484Q1, G142D, E154K L452R, D614G, P681R when compared with reference strain NC_045512.2. The present study is the first major genome analysis of SARS-CoV2 in Iraq. The data from this research could provide insights into SARS-CoV2 evolution, and can be potentially used to recognize the effective vaccine against the disease.