Abstract : Workload and work stress with fatigue are some of the risk factors for work performance problems. This study aims to determine the effect of workload, work stress’s and fatigue and their impact on the performance of nurses at Dadi Hospital, South Sulawesi Province. The research was conducted at the Dadi Hospital in South Sulawesi Province. This research uses quantitative methods. The population in this stuwasere 233 nurses at the Dadi General Hospital in South Sulawesi Province with 147 nurses used as samples. The type of research in this study is Analytical Observational using Cross-Sectionalal approach and this research uses a Path Analysis model, in which the intervening variable is fatigue which lies between exogenous variables. The data that has been collected is then analyzed through a process of data reduction, data presentation, and drawing conclusions and verification. From the results of the study it can be concluded that 46 respondents (31.3%) experienced heavy workload, 82 respondents (55.8%) experienced moderate work stress, 105 respondents (71.4%) were very tired), and poor work performance of 59 respondents (40.1%). Based on the results of statistical tests using the chi-square test, thp-valueue was 0.007 <0.05, meaning that there is a significant relationship between work stress and fatigue. And based on the results of AMOS work stress affects performance through fatigue (0.011). From the results of the study it can be concluded that 46 respondents (31.3%) experienced heavy workload, 82 respondents (55.8%) experienced moderate work stress, 105 respondents (71.4%) were very tired), and poor work performance of 59 respondents (40.1%). Based on the results of statistical tests using the chi-square test, thp-valueue was 0.007 <0.05, meaning that there is a significant relationship between work stress and fatigue. And based on the results of AMOS work stress affects performance through fatigue (0.011). From the results of the study it can be concluded that 46 respondents (31.3%) experienced heavy workload, 82 respondents (55.8%) experienced moderate work stress, 105 respondents (71.4%) were very tired), and poor work performance of 59 respondents (40.1%). Based on the results of statistical tests using the chi-square test, thp-valueue was 0.007 <0.05, meaning that there is a significant relationship between work stress and fatigue. And based on the results of AMOS work stress affects performance through fatigue (0.011). and poor work performance as many as 59 respondents (40.1%). Based on the results of statistical tests using the chi-square test, thp-valueue was 0.007 <0.05, meaning that there is a significant relationship between work stress and fatigue. And based on the results of AMOS work stress affects performance through fatigue (0.011). and poor work performance as many as 59 respondents (40.1%). Based on the results of statistical tests using the chi-square test, thp-valueue was 0.007 <0.05, meaning that there is a significant relationship between work stress and fatigue. And based on the results of AMOS work stress affects performance through fatigue (0.011).