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Abstract : Local anesthesia (LA) is the most commonly method for pain control during dental procedures. Dentists should have the knowledge, skills and attitude about LA. Therefore, this study aimed at evaluating online course about dental pain management and local anesthesia methods used in dentistry. A total of fifty-four dental students who accepted to participate in this study were included. An online course composed of six-module was developed. Students' knowledge was assessed by comparing their learning before and after their participation using identical pre and post-test. According to Chi-Square statistics, significant difference at P < 0.05 was found between the correct answers of participants in pre-tests and post-tests which indicates that the level of learning was significantly higher after the participation of the online course. The use of online educational course is effective in developing dental students' knowledge of dental pain management and the local anesthesia methods used in dentistry.