Nephrotoxicity is a great healthcare challenge in clinical care settings. Kidneys are subject to meteors from different chemicals, drugs, and xenobiotics; which make them susceptible to injury, among these are aminoglycoside antibiotics. A wide variety of therapeutic remedies has been used to protect kidneys against the toxicity of various drugs and chemicals including, vitamins, minerals, and herbal products. Beta carotene can decrease oxidative stress, so it can protect the kidney from the toxicity and damage induced by oxidative stress. In this study, we tried to investigate the possible ameliorative effect of beta carotene against amikacin-induced nephrotoxicity when given before and during the treatment of amikacin. Twenty-eight male albino rats were divided into five groups (seven in each group): 1-Control group (A): was intended for distilled water during the study period. 2-Amikacin group (B): were injected intraperitoneally with amikacin (150mg/kg/day) for two weeks. 3-Beta carotene group (C): were gavaged with beta carotene orally (100mg/kg/day) for 9 days. 4-Beta -carotene and amikacin group (D): were gavaged with beta carotene orally (100mg/kg/day) for 9 days alone, then injected intraperitoneally with amikacin (150mg/kg/day) and gavaged with beta carotene orally (100mg/kg/day) for two weeks. At the end of the study, animals were fasted and sacrificed, and serum was used for biochemical analysis and kidneys for histopathology. The results showed an elevation in serum MDA significantly (P < 0.005) and a reduction in T-AOC and GSH significantly (P < 0.005) in the amikacin group compared to the control group, whereas groups administered beta carotene with AMK showed significant improvement in the oxidative parameters compared to the AMK-treated group. In conclusion, beta carotene can act as a good antioxidant, preventing the expected nephrotoxicity induced by AMK.