Anal trauma is a very rare condition. Although rare, anal trauma has the potential to cause anal stenosis, ectropion, or incontinence that can interfere with a person's quality of life. This problem can also be caused after surgery on the anus, which is the primary modality of this case. More severe complications can include peritonitis, sepsis, to death. Therefore, an effective non-invasive alternative therapy is urgently needed in healing anal trauma. This research was conducted using an experimental laboratory method on male Wistar rats using a post-test control group consisting of three groups treated with PRP+βSVF (each, n=4), three groups treated without PRP+SVF (each, n=4), and one control group (sacrifice on day 0). All the treatment groups were sacrificed on days 1, 7, and 14 post-treatment. The anal trauma was made through a longitudinal incision from distal to proximal with a depth of 5 mm from serosa to intraluminal. TGF-β was measured using the ELISA method. Data represented the mean +/- standard deviation and was further analyzed by the one-way Anova test. There was a significant difference between the mean TGF- β levels in the intervention group and the control group on day 1 (31.07±1.65 vs. 25.6±1.02, p=0.01) and day 14 (31.47±0.51 vs. 27.42±1.17, p= 0.01). Meanwhile, on day 7, there was no significant difference (29.37±2.27 vs. 26.44±2.61, p=0.18). A combination of SVF and PRP effectively accelerates the healing process of anal trauma in vivo, which is assessed based on TGF-β levels.