Molybdenum oxides nanomaterials are gaining mounting popularity with potential applications in various fields due to their unique physicochemical properties. Making a nanocomposite is amended the properties of the original nanoparticles (NPs). In the present study, molybdenum dioxide/Graphene oxide /Chitosan nanocomposites (CS/GO-MoO2-NPs) were prepared using a chemical method and used for the extraction of precious proteins from human serum. CS/GO-MoO2-NPs were used as a surface to extract follicular-stimulating hormones (FSH) and luteinizing hormone (LH) from the human serum. The newly synthesized nanocomposite was characterized using routinely used techniques, including SEM, FTIR, and XRD measurements. Different concentrations of the hormone solutions were incubated with a fixed amount of NPs, and quantities of hormone absorbed were calculated. The studies were carried out at different temperatures to calculate the thermodynamic parameters. Furthermore, the composites were incubated with the human serum to extract the FSH and LH hormones and eluted the desorbed hormone. The results revealed the formation of stable slabs of CS/GO-MoO2-NPs with an average size of 23.5 nm. The nanocomposite can adsorb significant amounts of the FSH and LH hormones (7.07ug/g) from human serum. Freundlich's adsorption isotherm was obeyed during the adsorption process, showing that the nanocomposite surface was heterogeneous. Exothermic and spontaneous adsorption processes were revealed by thermodynamic research. FSH and LH hormones can be adsorbed spontaneously on the surface of CS/GO-MoO2-NPs as a tool for extracting hormones from serum and as a supporting media for FSH and LH in analytical kits such as ELISA and protein microarrays.