Methicillin-Resistant Staphylococcus aureus (MRSA) can cause infection with higher morbidity and mortality. In the limited-resource setting, the availability of rapid detection is doubtful; therefore, prompt detection using the alternative method is indispensable. To compare the detection rate of the modified-conventional method and chromogenic media against mucocutaneous clinical swab, during 12-month periods of study. A total of 80 S. aureus isolates from previous study were cultivated and re-cultured into routine media such as blood agar and mannitol-salt agar (MSA) between June and September 2018. It directly inoculated from plain blood agar that had been incubated one day before; it further underwent inoculation to other media, such as chromogenic media, blood agar and mannitol salt agar that had been supplemented with cefoxitin powder manually. The diagnostic value was obtained, such as the sensitivity of MSA-CFOX and BA-CFOX was 96.88%; and for CHROMagar and MHA-CFOX was 90.62%. The specificity of each MHA-CFOX, MSA-CFOX, and CHROMagar is 87.5% as well as BA-CFOX is 93.75%. The study has demonstrated better performance of the modified-conventional method compared to the other media. Hence, the application of modified-media should not be delayed to facilitate the findings of MRSA among hospitalized patients.