Abstract : Medroxyprogesterone is a synthetic progestogen (Progestin) derivative released as a contraceptive. Medroxyprogesterone and other progestins are suspected to negatively influence lipid level by various mechanisms. The objective of this research is to study the consequences of 8 weeks of exposure of albino rats to medroxyprogesterone on lipid profile. All experiments were ethically approved. This study was conducted on a treated group and a control group of female rats; each group includes 10 rats. The first group to compare the effects of medroxyprogesterone before and after use. The second is a weight-matched control group of albino rats which were not exposed to hormonal contraceptives to ensure that there was no change in lipids level during the study period by testing these parameters at the start and end of the study. The lipid profile investigation showed statistically significant changes in the treated rats before and treatment in all lipid parameters, including total cholesterol (TC) (118±7 mg/dl vs 128±5 mg/dl, p<0.01), low-density lipoprotein (45±5 mg/dl vs 59±4 mg/dl, p<0.001), very low density lipoprotein ( 23±1 mg/dl vs 24±1 mg/dl, p<0.05), high-density lipoprotein (HDL) (51±3 mg/dl vs 45±2 mg/dl, p<0.001), triglycerides (114±6 mg/dl vs 122±5 mg/dl, p<0.05), and TC/HDL (2±0.2 vs 2.9±0.1 p<0.01) compared to nonsignificant changes in the control group. The findings of this study confirm that the use of medroxyprogesterone could predispose to future negative effects as a result of high levels of all lipids, except for the low level of HDL, in the serum. Such negative effects may include cardiovascular problems like ischemic heart disease and cerebrovascular accidents.