Abstract : Single nucleotide polymorphisms (SNPs) play a crucial role in distinguishing disease-prone individuals. Identifying SNPs that affect protein stability is crucial. IRAK1 is one of the genes associated with Systemic Lupus Erythematosus susceptibility (SLE). This study aimed to identify nsSNPs within the IRAK1 gene that influence protein structure and function as a risk factor for SLE disease. Several in silico servers were used, including SIFT, PolyPhenv2, PROVEAN, SNAP, PhD-SNP, and Panther. Furthermore, nsSNPs identified as potentially harmful were investigated further using I-Mutant. The GeneMania server was used to identify gene interactions in order to determine whether the effect of existing polymorphisms was due to interactions with other genetic factors. Four nsSNPs with missense mutations in the human IRAK1 gene were identified: rs10127175, rs11465830, rs1059702, and rs1059703. Using the SIFT server, each of the four nsSNPs is tolerant. The Polyphen server's results demonstrated that the four SNPs were all benign. According to server data from Provean, four SNPs were all neutral. The SNAP server resulted one SNP to have no effect and three others to have an effect. Four SNPs on the PhD-SNP server were neutral. The Panther server yielded four SNPs all likely benign. According to the I-Mutant server analysis, four SNPs have the potential to reduce protein stability (decrease). There are nsSNPs of the IRAK1 gene, rs10127175, rs11465830, rs1059702, and rs1059703, that impair protein stability and are risk factors for SLE disease.