Abstract : Gentamicin one of Aminoglycoside antibiotics which is worldwide used to treat different microbial infection. Gentamicin cause various side effect in the recipient specially nephrotoxicity and ototoxicity as its reported by many studies. This drug causes higher damage in testicular tissues. The Prosopis fracta (PF) is a plant which has been used since ancient times as a type of traditional healing medicine in many parts of the world. The objective of this study include searching for the effectiveness of this plant in protection and / or ability of it in reduce the toxic effect of gentamicin in testicular tissues. The study performed on twenty-four Wistar male rats, weighing 200-250 gm. These rats divided into four groups: control group which kept on normal saline; group I provide gentamicin 100mg/kg, group II Low PF Dose (LPcD) which provide with same dose of gentamicin plus 100mg/kg of Prosopis fracta extract and group III High PF Dose (HPcD) which provide with same dose of gentamicin plus 200mg/kg of Prosopis fracta extract. After 10 days of the study all animals sacrificed and the testicular tissues blocks which prepared by paraffin method studded using H&E stain and immunohistochemical stain using Bcl-2 antibody. Gentamicin group G1 showed highly histopathological change including hyperplasia of leyding cells, necrosis of sertoli and spermatogenic cells, pykonotic nucleus of spermatogenic cells, deposition of acidophilic materials between tubules and necrosis of seminal tubules, congestion of blood vessels and increase thickness of tunica albuginea. In addition to that, this group showed strong positive reaction with the Blc-2 antibodies comparing with the control group. The third group G2 (LPcD) which provided with low dose of Prosopis fracta extract showed deposition of acidophilic materials, hyperplasia of Leydig cells, vacuolar degenerating in spermatogenic cells with little increasing in tunica albuginea thicknesses. The Blc-2 antibodies reaction showed weak positive result. The last group of the study G3 (HPcD) showed less histopathological changes and the group result of Bcl-2 reaction reveal to weak positive. The study confirms the histological toxicity of Gentamicin in testicular tissue and the positive result of Blc-2 ab reveal the ability of this drug in causing hyperplasia, pre tumor and apoptosis in testicular cells also the study elucidated the Prosopis fracta extract role in protection and curtailing of gentamicin toxicity effect in testicular tissue.