Selenium oxide nanoparticles (SeO2-NPs) have gotten much attention because of their favourable in addition to unique bioactivities. The use of nanocomposites for the extraction of precious proteins is an important field of study. The present work has introduced a new technique for synthesizing selenium oxide nanoparticles (SeO2-NPs) coated with chitosan and graphene oxide nanocomposites (CS/GO-SeO2-NPs). CS/GO-SeO2-NPs were used as a surface to extract follicular-stimulating hormone (FSH) from the human serum. The newly synthesized nanocomposite was characterized using routine techniques, including scanning electron microscopy (SEM), Fourier transition infrared spectrum (FTIR), and X-ray diffractometer (XRD) measurements. Different concentrations of the hormone solutions were with incubation a fixed the amount of NPs and the amount of hormone absorbed was computed using these numbers. To calculate the thermodynamic parameters, the experiments were carried out at various temperatures. Furthermore, the composites were incubated with the human serum to extract the FSH hormone and eluted the desorbed hormone. The results reveal the presence of stable flakes of CS/GO-SeO2-NPs with a 14.7 nm average size. The nanocomposite can adsorb significant amounts of the FSH hormone (7.07ug/g) from human serum. Freundlich's adsorption isotherm was followed during the adsorption phase, showing that the nanocomposite surface was heterogeneous. Exothermic and spontaneous adsorption processes were revealed by thermodynamic research. FSH hormone can be adsorbed on the surface of CS/GO-SeO2-NPs spontaneously as a tool for extracting hormones from serum and as a supporting media for FSH in the analytical kits.